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Antibacterial mechanisms of ZnO@PEG200 QDs against Uropathogenic E. coli CFT073. (A) Zeta potential analysis of electrostatic interaction between ZnO@PEG200 QDs and bacterial surfaces. (B) Zn 2+ concentrations released by ZnO@PEG200 QDs at pH = 7.4 (C) Membrane fluidity disruption assessed by ANS fluorescence. (D) Agarose gel electrophoresis of CFT073 DNA after ZnO@PEG200 QD treatment. (E) DCFH-DA assay for intracellular ROS generation. (F) Dose-dependent O₂ − production measured by absorbance. (G) Relative mRNA expression of virulence genes (FimH, FliC, papG, gapA, FimA, motA) in CFT073, normalized to nusA, showing downregulation of adhesion/motility genes.

Journal: International Journal of Pharmaceutics: X

Article Title: Precision PEGylation of ZnO quantum dots enables selective intracellular killing of Uropathogenic E. coli via multimodal antibacterial mechanisms without inducing resistance

doi: 10.1016/j.ijpx.2025.100479

Figure Lengend Snippet: Antibacterial mechanisms of ZnO@PEG200 QDs against Uropathogenic E. coli CFT073. (A) Zeta potential analysis of electrostatic interaction between ZnO@PEG200 QDs and bacterial surfaces. (B) Zn 2+ concentrations released by ZnO@PEG200 QDs at pH = 7.4 (C) Membrane fluidity disruption assessed by ANS fluorescence. (D) Agarose gel electrophoresis of CFT073 DNA after ZnO@PEG200 QD treatment. (E) DCFH-DA assay for intracellular ROS generation. (F) Dose-dependent O₂ − production measured by absorbance. (G) Relative mRNA expression of virulence genes (FimH, FliC, papG, gapA, FimA, motA) in CFT073, normalized to nusA, showing downregulation of adhesion/motility genes.

Article Snippet: The Bacterial DNA Genome Kit was obtained from Sangon Biotech (China).

Techniques: Zeta Potential Analyzer, Membrane, Disruption, Fluorescence, Agarose Gel Electrophoresis, DCFH-DA Assay, Expressing